An introduction to metabolic and cellular engineering by S Cortassa; et al

By S Cortassa; et al

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G. enzymes (depicted as double arrows on top layer). g. g. , 2001) (see text for further explanation). 6, we must consider the regulatory circuitry required for gene expression, involving transcriptional activators, suppresors, cis or trans-acting factors, most of them consisting of proteins, or DNA-binding proteins (Fig. 6, top layer). , bacteria, yeast) grown on agar plates. g. when grown with rich undefined media). Several reported data show that large variation of fluxes in metabolism can be achieved through a change in growth conditions, even at a constant growth rate.

G. product inhibition, effector activation). Thus, the dynamic behavior of metabolic networks cannot be assessed by these type of models. In Chapters 4 and 5 we introduce some basic concepts and tools for dealing with the dynamics of biological processes. Under homeodynamic conditions, living systems spatio-temporally coordinate their functioning by essentially top-bottom or bottom-up mechanisms. The former are represented by circadian and ultradian rhythms with clock characteristics whereas the latter emerge from the intrinsic, autonomous dynamics of the integrated mass-energy-information-carrying networks that represent living systems (Fig.

2000). Morphology and organization, like other complex phenotypes, are the outcome of interactions among the expression of multiple 18 An Introduction to Metabolic and Cellular Engineering genes and the environment. The relationship of genes to cell form and function is not like that of genes to proteins. , 1987; Harold, 1990; Aon and Cortassa, 1997). , 2001). e. the entire complement of messenger RNA (mRNA) molecules, proteins or metabolites in a tissue, organ or organism varies with physiological, pathological or developmental conditions (Oliver, 2000).

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