Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad by William S. M. Wold, Ann E. Tollefson

By William S. M. Wold, Ann E. Tollefson

Adenovirus tools and Protocols, moment version, now in volumes, is a vital source for adenovirus (Ad) researchers starting within the box, and an inspirational start line for researchers seeking to department into new parts of advert learn. as well as updating and increasing very important chapters from the 1st version, the authors have extra new chapters that tackle leading edge, fascinating parts of emphasis in advert study, together with advert vector development and use, real-time PCR, use of recent animal versions, and strategies for quantification of advert virus or virus expression/interactions. all of the protocols provided in those volumes is written through trendsetting researchers of their respective components of craftsmanship. quantity 1 addresses a number of very important options for building of adenoviruses to be used as vectors and for simple examine. Highlighted issues comprise deletion mutants, capsid changes, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In quantity 2, the authors specialise in tools that elucidate and quantitate the interactions of advert with the host. all the protocols in those volumes offers a common creation, via tried-and-true step by step tools. either beginner and skilled researchers will achieve super take advantage of those groundbreaking volumes in advert examine.

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Extra resources for Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

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Acad. Sci. USA 93, 3352–3356. 9. , Ho, W. , and Ornelles, D. (1995) Adenovirus type 5 precursor terminal protein-expressing 293 and HeLa cell lines. J. Virol. 69, 4079–4085. 10. , Weber, J. , and Massie, B. (2000) Proteasedeleted adenovirus vectors and complementing cell lines: potential applications of single-round replication mutants for vaccination and gene therapy. Hum. Gene Ther. 11, 1341–1353. 11. Zhang, W. and Imperiale, M. J. (2003) Requirement of the adenovirus IVa2 protein for virus assembly.

31 32 Groitl and Dobner sequently, the integrity of the recombinant viruses is verified by restriction digestion and DNA sequencing. 2. Materials 1. 0, per liter. Store at room temperature. 2. 6% Agarose in 1X TBE for gel electrophoresis. 3. Ethidium bromide stock solution (10 mg/mL): store at 4°C. 4. 0: autoclave and store at room temperature. 5. 0, and sonicate until the viscosity of the solution no longer changes appreciably. 0. Store at –20°C. 6. 5 M CaCl2: filter-sterilize and store at –20°C.

Virol. 70, 6497–6501. Defective Adenovirus Deletion Mutants 27 4. Krougliak, V. and Graham, F. L. (1995) Development of cell lines capable of complementing E1, E4, and protein IX defective adenovirus type 5 mutants. Hum. Gene Ther. 6, 1575–1586. 5. , Jia, X. , and Finer, M. H. (1995) A packaging cell line for propagation of recombinant adenovirus vectors containing two lethal gene-region deletions. Gene Ther. 2, 775–783. 6. , Dedieu, J. , and Perricaudet, M. (1996) Efficient dual transcomplementation of adenovirus E1 and E4 regions from a 293-derived cell line expressing a minimal E4 functional unit.

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